7. How is genetic engineering
used to create bacteria
capable of producing human
insulin?

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In the production of insulin by
bacteria the insulin geneis
incorporated into genetic material of
these . The mutant
bacteria multiply lineages of
insulin-producing bacteria.

bacteria there are circular of
DNA called plasmids,
which act as an to the main
DNA. create a mutant bacteria
of producing insulin a is
submitted to the of restriction
enzymes (restriction )
specialized in cutting DNA .
The once circular plasmid open by the
restriction . The same enzyme is
to cut a human molecule
containing the insulin . The piece of
human containing the insulin gene
has its extremities bound the
plasmid with the of DNA ligases.
The plasmid containing the
human gene is then inserted
the bacteria.

Another human already
produced by recombinant is GH
(somatotropin, or hormone).

The insertion of molecules into
cells of individual is also the
of the gene therapy, promising
treatment for genetic . Ingene
therapy cells from organism deficient
in the of a given protein
(by means of vectors,e.g.,
) pieces of DNA containing
protein geneand they then to
synthesize the protein.